Rice plays a crucial part in Cambodia’s agricultural landscape, serving as a primary food source for the population. The limited water in dry season becoming an increasing concern in the region,optimizing irrigation methods is to modify water consumption in rice production. This study was conducted to investigate the effect of three irrigation methods on the grain yield and water use efficiency of rice crops in soil columns under a nethouse with glass roof. A field experiment was conducted at the Cambodia Agriculture Research and Development Institute (CARDI), Cambodia. Rice was planted in February 2023 under different three irrigation methods. Three  irrigation regimes were examined during the growing period as follows: shallow deep shallow irrigation (T1), shallow frequency irrigation (T2),and continuous soil field capacity with irrigation depth at 10 mm daily (T3). Plant parameter and water amount data were collected during the growing period to evaluate the total yield and water use efficiency (WUE) of rice. The total average irrigation depth of different irrigation methods were 1050.67, 830.69 and 616.8 mm for T1, T2 and T3,repsepectively. According to results of effect on grain yield and WUE were found significant difference. Both grain yield  and WUE of T1 treatment obtained the highest value followed by T2,and T3 treatment, respectively.